Every office has its own protocol for who is called by his or her first name and who is called by his or her title. New employees should follow suit, after listening carefully to how people are addressed.
If yours is a "title" office, but you call your boss Charlie when meeting alone, you should still call him Mr. Dodd when others are around.
If yours is an informal office, you still should wait for the other person to say, "Please call me Jim," before doing so, if he has been introduced to you as "Mr. Culyer."
Everyone, no matter whether the office is formal of informal, has a name. No assistant should ever be referred to as "my girl." She is, if a possessive must be used, "Charlene Walter, my assistant," or "Angela Badalato, my assistant."
无论公司是否有无规矩，每个人都有称呼。不是每个经理助理都可以用类似“姑娘”的倪称。如果必须说明所属关系，则应如此介绍：她是Charlene Walter，我的助理，或我的助理 Angela Badalato。